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. 2001 Apr 10;98(9):4950–4954. doi: 10.1073/pnas.091601198

Figure 1.

Figure 1

Randomized MGMT library used for competitive BG and BCNU selection. The specific substitutions in each randomized sequence are shown on the left. Mutants are arranged in order of their frequency after selection. The number in parentheses identifies the MGMT mutant. All mutants were obtained originally from BG and MNNG selection in E. coli except MGMT-9, MGMT-10, and MGMT-18, which were selected in MNNG alone. MGMT-17 is the wt MGMT sequence and has no substitutions. The table on the right shows the frequency of recovery for each mutant. Pooled K562 cells expressing individual mutants were selected in high-stringency conditions (highest dose of 800 μM BG plus 20 μM BCNU) or low-stringency conditions (highest dose of 50 μM BG plus 20 μM BCNU). The column labeled “No selection” represents the baseline frequency in the library transfected into the retroviral producer cells. Although the library was slightly biased toward wt MGMT, each of the mutants was represented equally.