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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: Anal Biochem. 2012 Feb 8;423(2):241–245. doi: 10.1016/j.ab.2012.01.031

Fig. 1.

Fig. 1

Comparison of the calibration curves for the Western blot method (upper) and the dot blot method (lower). Lanes of the 14% SDS-PAGE reducing gel were loaded with varying amounts DNPH derivatized oxidized glutamine synthetase standard. From left to right, the load was 4.1, 2.0, 1.0, 0.50, 0.25, and 0.13 pmol carbonyl. The graph shows the averaged results from 2 separate blots. The regression line was fit to all points, giving y=26.6x −1.4, r2>.99. The dot blot samples were spotted in triplicate, with the top row containing no protein. All other rows were loaded with 60 ng glutamine synthetase. The carbonyl content from top to bottom was 0.13, 0.30, 0.35, 0.46, 0.57, and 0.79 pmol. The equation of the fit line is y = 16.3x + 7.7, r2>.99 with 3 measurements for each point.