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. Author manuscript; available in PMC: 2012 Mar 28.
Published in final edited form as: J Cancer Ther. 2011 Jun;2(2):77–90. doi: 10.4236/jct.2011.22009

Figure 2.

Figure 2

Increases in caspase-3 activity and apoptotic populations in SK-N-BE2 and SH-SY5Y cells. Treatments: CTL, 4-HPR (0.5 μM for 72 h), GST (100 μM for 24 h), and 4-HPR (0.5 μM for 72 h) + GST (100 μM for 24 h). (A) Determination of caspase-3 activity using a colorimetric assay. (B) Annexin V-FITC/PI staining and flow cytometric determination of apoptosis. Treatment with 4-HPR + GST effectively controlled growth of neuroblastoma cells due to increase in caspase-3 activity for apoptosis.