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. Author manuscript; available in PMC: 2012 Mar 28.
Published in final edited form as: Acta Neuropathol. 2011 Nov 6;123(1):119–132. doi: 10.1007/s00401-011-0898-8

Fig. 3.

Fig. 3

Selective tau tyrosine nitration in PSP. a Tau-nY18 did not label the pathological inclusions associated with PSP. b Tau-nY29 reacted with the globose tangles and with c the perinuclear inclusions but d, e Tau-nY197 localized to the neuropil threads following CIP treatment. f Tau-nY394 did not label any of the pathological lesions associated with PSP. g Tau-Y197 reacted with numerous neuropil threads and h globose tangles (asterisk) as well as i the thorny (asterisk) and tufted astrocytes associated with PSP. j By Western blot analysis Tau-nY18 did not react with the soluble tau but did label the insoluble fractions whereas Tau-nY29 labeled soluble tau but not insoluble fractions. Tau-nY197 labeled both the soluble and insoluble fractions, while Tau-nY394 did not react with any fractions analyzed. k Abundant soluble tau was detected with RD3, RD4 and Tau-12. The insoluble fractions, however, were only labeled with the Tau-12 antibody. In all panels, calibration bars represent 20 μm