Skip to main content
. 2012 Apr;76(2):149–156.

Table I.

Statistical probabilities supporting differences in the proportions of peripheral blood cell subsets in heifers classified as having a high antibody-mediated/low cell-mediated (HiAMIR) or low antibody-mediated/high cell-mediated (HiCMIR) immune response phenotype before (day 0) and after (day 7) secondary immunization

Factor1
Ptype Ptype*Age Ptype*Age*Age Ptype*Status
Cell phenotype2 Time3 HiAMIR4 HiCMIR4 Ratio/Difference5 P-value P-value6 P-value6 P-value6
% of CD45+ lymphocytes
 aWC1+ day 0 17.6 22.2 0.750 (0.578 to 0.972) 0.031 NS NS NS
 aWC1+ day 7 22.4 20.2 1.139 (1.008 to 1.287) 0.038 NS NS NS
 bCD4+ day 0 26.2c 25.0c 1.221 (−1.310 to 3.752) 0.332 0.042 0.044 NS
 bCD4+ day 7 25.9 27.1 1.263 (−0.582 to 3.108) 0.172 NS NS NS
 aCD8+ day 0 13.3c 12.6c 1.061 (0.905 to 1.244) 0.452 0.024 0.033 NS
 aCD8+ day 7 11.3 12.1 0.927 (0.843 to 1.020) 0.116 NS NS NS
 aIgM+ day 0 26.3 26.1 1.008 (0.825 to 1.231) 0.938 NS NS NS
 aIgM+ day 7 28.6 26.7 1.102 (1.003 to 1.211) 0.044 NS NS NS
 aCD5+ day 0 73.0 73.5 0.972 (0.803 to 1.176) 0.764 NS NS NS
 aCD5+ day 7 73.1d 73.3d 0.986 (0.897 to 1.085) 0.769 NS NS NS
% of total leukocytes
 aMonocyte day 0 4.5 5.3 0.840 (0.692 to 1.019) 0.075 NS NS 0.066
 aMonocyte day 7 4.2 3.9 1.094 (0.881 to 1.358) 0.405 NS NS 0.050
 aLymphocyte day 0 40.8c 46.6c 0.790 (0.618 to 1.009) 0.058 0.006 NS 0.030
 aLymphocyte day 7 67.4c 68.0c 0.976 (0.836 to 1.139) 0.748 0.062 0.065 0.051
 aNeutrophil day 0 26.6 25.7 1.047 (0.877 to 1.251) 0.598 NS NS NS
 aNeutrophil day 7 28.0c 26.0c 1.108 (0.855 to 1.437) 0.423 0.005 0.025 0.056
 aEosinophil day 0 4.7c 3.4c 1.376 (0.947 to 1.999) 0.091 0.040 0.041 NS
 aEosinophil day 7 1.8c 1.8c 0.993 (0.788 to 1.252) 0.952 0.091 0.080 NS
1

Factor: Ptype — phenotype classification; Status — pregnancy status; A*B — interaction between factor A and B.

2

Data logit transformed a or did not require transformation b for analysis.

3

Time: day 0 — before immunization; day 7 — 7 d after immunization.

4

Values represent least squares means (LSMs) or back-transformed geometric LSMs (transformed variables); Where interactions of Ptype and age c or Ptype and day 0 covariate d were included in the model, Ptype LSMs were estimated at the mean age of heifers or mean day 0 value respectively.

5

Values represent the difference between Ptype LSMs or the back-transformed ratio of Ptype LSMs (transformed variables); Numbers in brackets indicate 95% confidence interval.

6

NS — factors removed from model statement (P > 0.1).