Skip to main content
. 2012 Mar 26;122(4):1339–1353. doi: 10.1172/JCI57256

Figure 4. JNK mediates AβO-induced IRS-1pSer.

Figure 4

(A) Representative image showing low IRS-1pSer levels (red) in a hippocampal neuron transfected with GFP-fused DN JNK (green; scale bar: 50 μm). (B) Higher-magnification image of dendrite segment (white box in A; scale bar: 10 μm). (C and D) integrated IRS-1pSer636 and IRS-1pTyr465 immunofluorescence levels, respectively. *P < 0.001 relative to vehicle-treated cultures, #P < 0.001 relative to AβO-exposed cultures; ANOVA followed by Bonferroni post-hoc test. (E–H) Hippocampal neurons exposed for 3 hours to vehicle (E), 500 nM AβOs (F), 10 μM SP600125 (SP) plus 500 nM AβOs (G), or 1 μg/ml infliximab (Inflix) plus 500 nM AβOs (H). Scale bar: 50 μm. (I) Integrated IRS-1pSer immunofluorescence levels determined from 4 experiments (independent cultures, 20 images analyzed/experimental condition/experiment). *P < 0.001 relative to vehicle-treated cultures, #P < 0.001 relative to AβO-exposed cultures; ANOVA followed by Bonferroni post-hoc test. Scr, scrambled Aβ1–42 peptide. (J) Immunoblot of p-JNK in cultures exposed to 500 nM AβOs for 3 hours. (K) p-JNK levels in hippocampal homogenates from APP/PS1 Tg or WT mice. tJNK, total JNK. (L) TNF-α immunoblot in concentrated conditioned medium from cultures exposed to AβOs for 3 hours. (M and N) TNF-α receptor levels in cultures exposed to 500 nM AβOs for 3 hours and in hippocampal homogenates from APP/PS1 Tg (n = 7) or WT mice (n = 5), respectively. (M and N) Densitometric quantification normalized by cyclophilin B. Lanes in J and L–N were run on the same gel but were noncontiguous. *P < 0.02, Student’s t test.