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. 2012 Mar 12;122(4):1469–1486. doi: 10.1172/JCI57349

Figure 4. G9a forms a complex with Snail and DNMTs.

Figure 4

(A) HEK293 cells were transiently coexpressed with Flag-tagged G9a, HA-tagged Snail, and Myc-tagged DNMTs. Cell extracts were immunoprecipitated separately with Flag, HA, or Myc antibodies, and the associated G9a, Snail, and DNMTs were examined by Western blotting, respectively. (B) Endogenous Snail, G9a, and DNMTs were immunoprecipitated from MDA-MB157, BT20, and MDA-MB231 cells, and bound endogenous Snail, G9a, and DNMTs were examined by Western blotting. (C) Snail or NTC siRNA was expressed in MDA-MB157 cells, and after immunoprecipitation of endogenous G9a, bound Snail and DNMTs was subjected to Western blotting. (D) G9a or NTC siRNA was expressed in MDA-MB157 cells, or cells were treated with the G9a inhibitor BIX01294 (2.5 μM), and after immuno­precipitating endogenous Snail, bound DNMTs and G9a were subjected to Western blotting.