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. 2012 Mar 26;122(4):1541–1552. doi: 10.1172/JCI58765

Figure 1. Functional integrity of p53 pathway in HIM models.

Figure 1

Mice harboring WU-BC3, WU-BC4, and WU-BC5 were treated with vehicle (V) or irinotecan (I), and tumors were isolated 24 hours later. Lysates were prepared and resolved by SDS-PAGE and analyzed for the indicated proteins by Western blotting. Note the increased baseline p53 expression due to the missense mutation in WU-BC4 (lane 3) compared with WU-BC3 (lane 1) and the absence of intact p53 protein due to the deletion mutation in WU-BC5 (lane 5).