Figure 3. Resident LRCs.

Two months after neonates were injected with BrdU, LSECs were isolated by elutriation, and the CD133⁺ fraction was isolated by immunomagnetic separation to obtain resident SPCs and LRCs. (A) CD31 staining of resident SPCs and LRCs. (B) CD45 staining of resident SPCs and LRCs. (C) BrdU⁺ staining indicates LRCs (white arrows). (D) Differential interference contrast (DIC) demonstrates all cells in the field. (E) The merged image shows CD133⁺ cells that are CD31⁺CD45⁺BrdU⁺ LRCs (white), indicated by white arrows. Original magnification, ×100.