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. 2012 Mar 19;122(4):1246–1261. doi: 10.1172/JCI61135

Figure 5. L selectin ligands are activated in DBA/2J eyes but not in radiation-treated eyes.

Figure 5

The MECA-79 antibody binds to all known, functionally activated L selectin ligands and marks sites of chronic inflammatory signaling. (B, E, H, and K) MECA-79 bound to vessels in the ONH of DBA/2J eyes at early stages of glaucoma, prior to detectable optic nerve damage or axon loss. No MECA-79 staining was observed in the ONHs of either (C, F, I, and L) radiation-treated or (A, D, G, and J) D2-Gpnmb+controls. n = 6 eyes for each group. (H and K) MECA-79 staining coincided with an increase in staining for IBA1, a marker of microglia and monocyte-derived cells. Arrows indicate location of blood vessels, which are shown at higher magnification in the bottom panels (D–F and J–L). Scale bars: 50 μm (A–C and G–I); 20 μm (D–F and J–L).