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. 2012 Mar 1;122(4):1553–1566. doi: 10.1172/JCI61143

Figure 8. Dual infection of H. pylori strains expressing different single phosphorylatable or phosphomimetic EPIYA motifs induces AGS cell elongation.

Figure 8

AGS cells were infected for 4 hours with CagA EPIYAY>F (A) or EPIYAY>D (B) mutant strains as indicated. The available single phosphorylatable or phosphomimetic EPIYA motifs for each double infection are indicated. The number of elongated cells in each experiment was quantitated in triplicate in 10 different 0.25-mm2 fields, and CagA phosphorylation was examined using α–PY-99 and α-CagA antibodies (arrows). (C) AGS infection for 4 hours with the indicated CagA-expressing strains in the presence or absence of c-Src inhibitor PP2 (10 μM) or c-Abl inhibitor SKI-DV2-43 (1 μM) revealed significant changes in AGS cell elongation as quantitated in triplicate in 10 different 0.25-mm2 fields. *P ≤ 0.01; **P ≤ 0.001.