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. 2012 Mar 1;122(4):1529–1540. doi: 10.1172/JCI61350

Figure 3. FAK-cortactin dispersion is essential for cell rounding and enhanced radiosensitivity.

Figure 3

(A) Scheme of FAKwt-GFP, FAKca-GFP (K→E plus K→E at residues 578 and 581 [K578E/K581E]), and GFP empty vector controls (pEGFP-N1). (B) Western blot from whole cell lysates of UTSCC15 cells stably transfected with FAK-GFP fusion constructs indicated in A. β-Actin served as loading control. (C) Clonogenic survival of AIIB2-treated FAKca-GFP, FAKwt-GFP, and GFP transfectants (IgG#1 as control). Results show mean ± SD (n = 3; t test; **P < 0.01). (D) PLA using anti–β1 integrin and anti–FAK Y397 antibodies and confocal microscopy on AIIB2-treated UTSCC15 transfectants. DAPI was used for nuclear staining. Scale bar: 10 μm. (E) Clonogenic radiation survival of AIIB2-pretreated and irradiated (2–6 Gy) FAKca-GFP, FAKwt-GFP, and GFP transfectants. Results show mean ± SD (n = 3; t test; *P < 0.05; **P < 0.01). See also Supplemental Figure 15.