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. 2012 Mar 12;122(4):1503–1518. doi: 10.1172/JCI61392

Figure 6. Effect of genetic manipulation of VCAN in UMUC3 cells on their in vitro appearance.

Figure 6

(A) WB confirming the efficiency of Vcan knockdown/expression in UMUC3-GFP and UMUC3-GDI2 cells transduced with shRNA lentiviruses targeting all VCAN isoforms (shVcan), an irrelevant nontarget (NTsh), or retroviruses overexpressing V1, V3, and V2 and their empty vector controls (VC). Numbers represent the relative band density normalized to corresponding tubulin, with GFP-VC considered as 1. (B) Engineered UMUC3 cells in A were allowed to invade Matrigel-coated 8-μm inserts toward CGM or U937. *P < 0.05. Bars represent mean ± SEM of counted invading/attracted cells of 3 independent experiments performed in triplicate. Student’s t test was used.