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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Int J Biochem Cell Biol. 2012 Jan 10;44(4):648–658. doi: 10.1016/j.biocel.2012.01.002

Figure 7. In vitro metabolic function of ES cell-derived hepatocytes.

Figure 7

(A) GFP expression as the purification marker to enrich ES cells-derived hepatocytes (ESD-H) after sorting. (B) Glycogen storage in ESD-H was stained as purple by PAS staining. (C) ac-LDL transportation into ESD-H was analyzed by DiI-ac-LDL uptake assay (red staining). (D) Cellular uptake of ICG in ESD-H (green staining). (E) Cellular ammonia clearance was observed in ESD-H culture supernatant. (F) The levels of albumin protein levels secreted in ESD-H culture mediums were measured by ELISA assay. (G) Immunofluorescence staining of albumin and FAH in ESD-H. *: p < 0.05. Scale bars: 200 µm.