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Temperature dependence of the enthalpy of zinc binding for A* (■), A*K15A+E111 M (▴), A*I329F (▾), A2B1 (⊙), and EDTA (♦) and of maltose binding for MBP (●) as determined by isothermal titration calorimetry. The slope of these lines gives the observed heat capacities, ΔCp, (cal mol−1⋅K−1): MBP, −297; A*, −194; A*K15A+E111M, −184; A*I329F, −99; A2B1, −10; EDTA, −6.
