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Kinetics of ligand binding, as monitored by changes in fluorescence of IANBD in a stopped-flow apparatus, at 25°C. (a) MBP binds maltose with classic pseudo-first-order dependence on maltose concentration. (b) The binding of zinc to A* shows more complex behavior that is consistent with a two-state model in which formation of the closed state is rate-limiting (see text, Scheme 3, and Eq. 3).
