Fig. 5.

Blockade by anticholinesterase agents of AChE-evoked potentiation of [³H]–FW and [³H]–AMPA binding. Synaptosomal cortical membranes (A, C) and 15 μm brain sections (B) from adult male rats were exposed to different concentrations of AChE–BW284c51 for 30 min at 37 °C (synaptosomal membranes) or room temperature (sections). After this pretreatment, samples were extensively washed and submitted to either centrifugation binding assays (A) or autoradiography (B) experiments using 10 nM [³H]–FW. Fig. 5C shows inhibition by anticholinesterase agents of [³H]–AMPA binding potentiation on adult membranes. Cortical membranes were exposed to a mixture of 1 U ml⁻¹ AChE plus either buffer or 20 μM propidium iodide, 10 μM eserine or 1 μM DFP (final concentrations in the same buffer) for 30 min at 37 °C. After 2 washes, 25 nM [³H]–AMPA binding assay was carried out. Plots represent means±S.D. of specific radioactivities from three separate experiments, each one performed by triplicate. Significance level was P < 0.01.