Table 3.
1 DIV |
3 DIV |
5 DIV |
|||||||
---|---|---|---|---|---|---|---|---|---|
Control | AChE 0.01 U/ml |
AChE 0.05 U/ml |
Control | AChE 0.01 U/ml |
AChE 0.05 U/ml |
Control | AChE 0.01 U/ml |
AChE 0.05 U/ml |
|
SV2a | 23 ± 1 | 42 ± 2 | 38 ± 1 | 23 ± 9 | 33 ± 1 | 38 ± 4 | 40 ± 3 | 55 ± 8 | 66 ± 11 |
GluR2 | 11 ± 2 | 14 ± 3 | 28 ± 1 | 20 ± 8 | 21 ± 1 | 31 ± 9 | 28 ± 12 | 36 ± 7 | 47 ± 13 |
Colocalisation | 5 ± 0 | 10 ± 1 | 17 ± 2 | 17 ± 6 | 18 ± 1 | 23 ± 6 | 17 ± 1 | 25 ± 4 | 40 ± 9 |
Note. Postnatal hippocampal neurones from 1, 3, and 5 DIV were treated with 0.01 and 0.05 U/ml AChE every day for 5 days. The number of clusters of the presynaptic marker SV2a, for GluR2 and the extent of colocalisation (puncta immunostained with both antibodies), are shown per 100 μm of dendrite.