Figure 2. Cell bodies and synaptic structures of cultured embryonic neurons contain profilin 1 (PFN1) and profilin 2a (PFN2a).

Confocal images showing the simultaneous immunostaining of PFN1 and PFN2a in a mouse hippocampal neuron (DIV14) with monoclonal antibodies specific for each isoform, and with a rabbit serum specific for the synaptic markers synapsin 1 and 2. Note that both isoforms are concentrated in synapses (arrows, right panels). Bar: 10 µm.