Figure 6. BDNF treatment results in an enrichment of both profilin isoforms in synapses of hippocampal neurons.

(A): Rat hippocampal neurons at DIV9 were treated with BDNF or BSA as control (Co) for 22 hours, fixed and stained for the synaptic marker synapsin, PFN1 or PFN2a. Bar: 3 µm. (B): Quantitative analysis of the enrichment factor. 250 to 400 synapses were quantified in 3 independent experiments for each PFN isoform; mean ± SEM; paired t test; * P<0.05.