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. 2001 Apr 24;98(9):4961–4965. doi: 10.1073/pnas.091100698

Figure 2.

Figure 2

Inhibition of GDP/GTP exchange on ARF-1 by M69. (A) GDP/GTP-exchange activity of cytohesin 1, C1-Sec7, C2-Sec7, and Gea2-Sec7 on ARF-1 in the presence of M69 (solid bars) and unselected RNA library (shaded bars). The amount of bound [γ35-S]GTP after 30 min without RNA was defined as 100%. (B) Concentration dependence of inhibition of cytohesin 1-catalyzed GDP/GTP exchange on ARF-1 by M69 (■) and unselected pool (♦). (C) Time course of inhibition of cytohesin 1-catalyzed GDP/GTP exchange on ARF-1 by M69 (▴) vs. noninhibited negative control (■). (D) GDP/GTP-exchange activity of cytohesin 1, C1-Sec7, C2-Sec7, and Gea2-Sec7 on ARF-1 in the presence of BFA.