Table 1.
Kd of aptamer/protein interaction
| Aptamer |
Kd, nM*
|
|||
|---|---|---|---|---|
| Cytohesin 1 | C1-Sec7 | PH | G2-Sec7 | |
| M5 | 40.0 ± 8.0 | 36.0 ± 3.4 | 480 ± 50 | 48.0 ± 5.0 |
| M56 | 5.0 ± 1.0 | 280 ± 28 | 150 ± 8.0 | >1,000 |
| M69 | 16.0 ± 0.4 | 16.0 ± 0.3 | NA | NA |
Binding of radiolabeled aptamer clones to full-length cytohesin 1 and its subdomains C1-Sec7, PH, and C domain as well as to the large GEF yeast Gea2-Sec7 domain (G2-Sec7) was examined by filter-binding assays. All data were recorded in triplicate measurements. NA, no affinity.
*
The C domain peptide
NH
-CYEMLAARKKKVSSTKRH-COO− showed no
affinity for all three aptamers.