Table 1. Sequence, Tm and references of primers used.

PRIMERS	5′-3′ SEQUENCES	Bp	Tm(C°)	reference
MY09	CGTCCMARRGGAVVACTGATC	∼450	50	Manos et al 1989
MY11	GCMCAGGGVVCATAAYAATGG		52
GH20	GAAGAGCCAAGGACAGGTAC	260	62	Saiki et al 1988
PC04	CAACTTCATCCACGTTCA CC		60
16E6-S 26	AAGGGCGTAACCGAAATCGGT	207	64	Nagao et al 2002
16E6-AS 233	CATATACCTCACGTCGCAG		58
16E2-S 3383	TTATTAGGCAGCACTTGGCCA	177	62	Nagao et al 2002
16E2-AS 3560	GTGAGGATTGGAGCACTGTCC		64
16E2-S 2734	AGGACGAGGACAAGGAAAA	1139	56	Yoshinouchi et al 1999
16E2-AS 3846	GGA TGC AGT ATC AAG ATT TG		56

Names of HPV16 primers include sense of primer extension followed by the position of the 5′ nt on prototype sequence for rapid primer location.