Figure 5. Clonogenic potential progressively declines with increasingly differentiated state of H7 ES cell-derived lung cells.

Clonogenic potential was determined in single cell suspensions of undifferentiated H7 ES cells on days 1 and 7 of culture in ES cell medium (i.e., D1ES and D7ES), EBs on day 2 and 4 of culture in EB medium (i.e., D2EB and D4EB), cells from 6 and 12 days of culture in SAGM (i.e., D6SAGM and D12SAGM), and cells from 8 and 12 days in culture in BEGM (i.e., D8BEGM and D12BEGM). Colony forming assays were done with single cell suspensions from each stage in culture and performed in duplicate. Numbers represent colonies counted per 10⁵ cells plated in duplicate after 7 days in culture. The results represent counts from 3 independent experiments as mean ± SEM. P value<0.05 was considered significant, compared to colony forming unit (CFU)s from D1ES cells in undifferentiated state.