Table 1. Optimal conditions for BacMam transduction of transporter expression plasmids in HEK293-MSRII cells.

Transporter	Mode of Transdution	Pfu/ml	Final Seeding density (cells/cm2)	MOI	Max BacMam (%)
OATP1A2	In-tube	1.07×108	2.6×105	60	14
OATP1B1	In-well	1.78×108	2.1×105	180	20
OATP1B3	In-tube	1.54×108	2.1×105	155	20
OATP2B1	In-tube	6.80×108	2.6×105	40	1

Pfu = plaque forming units; MOI = multiplicity of Infection.