. 2001 Apr 24;98(9):4966–4971. doi: 10.1073/pnas.081424898

Table 1.

Membrane ATPase activities from cells expressing c₃ and c₄ fused subunits

Plasmids expressed	ATPase activity^*		Inhibition by DCCD^†
Plasmids expressed	−LDAO	+LDAO	Inhibition by DCCD^†
pWOc3(I30C)	0.61	3.48	73%
pWOc4(I30C)	0.81	4.40	70%
pWOc3(I30C)/pCOc4(I30C)	1.20	5.45	72%
pWOc4(I30C)/pCOc3(I30C)	1.45	7.10	55%

Membrane ATPase activity measured with and without 0.5% LDAO, which activates F₁ ATPase by disassociation of the inhibitory ɛ subunit. Activity expressed in units of μmol min⁻¹ mg⁻¹.

^†

Membrane ATPase activity was measured after preincubation of membranes at 10 μg/ml in ATPase assay buffer with 50 μM DCCD for 20 min at 22°C.