3.
Hematopoietic Differentiation in Nrarp−/− and Control Littermate Mice
| Tissue | Cell type | Stain | Nrarp+/+ | Nrarp+/− | Nrarp−/− |
|---|---|---|---|---|---|
| Thymus | Early T (DN1) | CD25−/CD44+/Lin− | 26.5 ± 4.6 | 31.4 ± 4.9 | 27.1 ± 3.2 |
| Early T (DN2) | CD25+/CD44+/Lin− | 8.8 ± 0.9 | 8.9 ± 0.5 | 9.3 ± 1.4 | |
| Early T (DN3) | CD25+/CD44−/Lin− | 35.9 ± 5.8 | 34.9 ± 7.0 | 38.1 ± 5.2 | |
| Early T (DN4) | CD25−/CD44−/Lin− | 28.8 ± 8.0 | 24.9± 3.8 | 25.5 ± 6.3 | |
| T (CD4) | CD4+/CD8− | 8.4 ± 1.0 | 8.5 ± 0.9 | 9.9 ± 1.2 | |
| T (DP) | CD4+/CD8+ | 87.2 ± 1.5 | 87.0 ± 0.9 | 85.0± 1.5 | |
| T (CD8) | CD4−/CD8+ | 2.5 ± 0.3 | 2.4 ± 0.2 | 2.7 ± 0.1 | |
| T (DN) | CD4−/CD8− | 1.9 ± 0.5 | 2.2 ± 0.4 | 2.4 ± 0.6 | |
| Spleen | T (CD4) | CD4+/CD8− | 20.4 ± 2.4 | 23.5 ± 3.2 | 23.7 ± 1.8 |
| T (DP) | CD4+/CD8+ | 0.5 ± 0.1 | 0.8 ± 0.4 | 0.6 ± 0.1 | |
| T (CD8) | CD4−/CD8+ | 9.0 ± 0.8 | 10.2 ± 1.6 | 9.9 ± 0.8 | |
| T (DN) | CD4−/CD8− | 70.1 ± 2.9 | 65.6 ± 5.0 | 66.0 ± 2.0 | |
| Myeloid | Mac1+ | 6.3 ± 0.3 | 6.9 ± 2.2 | 6.3 ± 1.1 | |
| B cells | B220+/CD19+ | 57.2 ± 3.2 | 57.8 ± 2.1 | 57.8 ± 3.1 | |
| Bone marrow | Myeloid | Mac1+ | 45.3 ± 4.4 | 44.2 ± 3.3 | 42.4 ± 5.7 |
| Erythroid | CD71+/Ter119+ | 26.2 ± 3.2 | 25.7 ± 2.2 | 26.0 ± 6.7 | |
| B cells | B220+/CD19+ | 26.3 ± 2.9 | 24.8 ± 4.0 | 28.9 ± 4.9 | |
| HSC | cKit+/Sca1+/Lin− | 2.9 ± 0.2 | 2.9 ± 0.6 | 2.5 ± 0.2 |
Abbreviations: DN, double negative; DP, double positive. For myeloid cells, erythroid cells, B cells, and hematopoietic stem cells (HSC), data are presented as percentage ± standard deviation of cells with the indicated marker expression profile. For early T cells (DN1–DN4) and T cells (CD4, DP, CD8, DN), data are presented as the percentage ± standard deviation of total early T cells (DN cells) or T cells, respectively. Cells were isolated from four mice from each of the indicated genotypes.