Table 1. Primers used for quantitative PCR.
Gene | Sequence 5′ → 3′ |
---|---|
TNFR1 | Forward: 5′- TGCCTACCCCAGATTGAGAA -3′ |
Reverse: 5′- ATTTCCCACAAACAATGGAGTAG -3′ | |
TNF-α | Forward: 5′- ACAAGCCCAACAACAAGG -3′ |
Reverse: 5′- ATCGGGATGCCAAAGAGG -3′ | |
FAS-R | Forward: 5′- AGCTTGGTCTAGAGTGAAAA -3′ |
Reverse: 5′- GAGGCAGAATCATGAGATAT - 3′ | |
FAS-L | Forward: 5′- CACTTTGGGATTCTTTCCAT -3′ |
Reverse: 5′- GTGAGTTGAGGAGCTACAGA -3′ | |
DR4 | Forward: 5′- CGATGTGGTCAGAGCTG-GTACAGC -3′ |
Reverse: 5′- GGACACGGCAGAGCCTGTGCCATC -3′ | |
DR5 | Forward: 5′- GGGAGCCGCTCATGAGGAAGTTGG -3′ |
Reverse: 5′- GGCAAGTCTCTCTCCCAGCGTCTC -3′ | |
GAPDH | Forward: 5′- ATGGGGAAGGTGAAGGTCGG -3′ |
Reverse: 5′- GACGGTGCCATGGAATTTGC -3′ |
Abbreviations: DR=death receptor; FAS-L=Fas ligand; FAS-R=Fas receptor; GAPDH=glyceraldehyde 3-phosphate dehydrogenase; TNF-α=tumour necrosis factor-α; TNFR1=tumour necrosis factor receptor-1.