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. 2012 Mar 1;106(7):1288–1296. doi: 10.1038/bjc.2012.66

Table 1. Primers used for quantitative PCR.

Gene Sequence 5′ → 3′
TNFR1 Forward: 5′- TGCCTACCCCAGATTGAGAA -3′
  Reverse: 5′- ATTTCCCACAAACAATGGAGTAG -3′
   
TNF-α Forward: 5′- ACAAGCCCAACAACAAGG -3′
  Reverse: 5′- ATCGGGATGCCAAAGAGG -3′
   
FAS-R Forward: 5′- AGCTTGGTCTAGAGTGAAAA -3′
  Reverse: 5′- GAGGCAGAATCATGAGATAT - 3′
   
FAS-L Forward: 5′- CACTTTGGGATTCTTTCCAT -3′
  Reverse: 5′- GTGAGTTGAGGAGCTACAGA -3′
   
DR4 Forward: 5′- CGATGTGGTCAGAGCTG-GTACAGC -3′
  Reverse: 5′- GGACACGGCAGAGCCTGTGCCATC -3′
   
DR5 Forward: 5′- GGGAGCCGCTCATGAGGAAGTTGG -3′
  Reverse: 5′- GGCAAGTCTCTCTCCCAGCGTCTC -3′
   
GAPDH Forward: 5′- ATGGGGAAGGTGAAGGTCGG -3′
  Reverse: 5′- GACGGTGCCATGGAATTTGC -3′

Abbreviations: DR=death receptor; FAS-L=Fas ligand; FAS-R=Fas receptor; GAPDH=glyceraldehyde 3-phosphate dehydrogenase; TNF-α=tumour necrosis factor-α; TNFR1=tumour necrosis factor receptor-1.