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. 2001 Apr 10;98(9):4978–4983. doi: 10.1073/pnas.081088798

Figure 1.

Figure 1

Characterization of the antibodies against the p60 subunit of Polɛ. (A) Western blotting of Xenopus egg extracts (0.5 μl) with the Polɛ p60 antibodies. (B) Coimmunoprecipitation of the catalytic subunit of Polɛ with the p60 antibodies. Immunoprecipitation from egg extracts with the p60 antibodies (αp60) (lanes 1 and 2) or with whole rabbit immunoglobulin G (control IgG) (lanes 3 and 4) was performed. The resultant supernatants (SUP, lanes 1 and 3) and precipitates (PPT, lanes 2 and 4) were analyzed by Western blotting with the p60 antibodies (Lower) or the antibodies against the catalytic subunit of Polɛ (Upper). Arrowheads indicate the catalytic subunit (p260) and p60 of Polɛ, respectively.