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Sequence characterization of CHO pre-miRNAs. (a) Conservation CHO (cgr) mir-17-92 pre-miRNAs in respect to Mus musculus (mmu); *, sequence matches; - sequence deletions. (b) PCR amplification of miRNA clusters: PCR amplification of miRNA clusters using genomic DNA from CHO-K1 and CHO dhfr (-) cells. Lanes 1–4 and 6–9 showing specific amplification for miR-24-23a (1/6), miR-17-92a, miR-221-222 and miR-24-23b. Lanes 5 and 10 no template control PCR.
