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. 2012 Mar 29;6:7. doi: 10.3389/fnbeh.2012.00007

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Copyright © 2012 Radley.

This is an open-access article distributed under the terms of the Creative Commons Attribution Non Commercial License, which permits non-commercial use, distribution, and reproduction in other forums, provided the original authors and source are credited.

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Top: Overlap of retrograde tracer injections in PVH (Fluoro-gold, FG; cyan), and anterograde tracers in PL (BDA; green) and vSUB (FlouroRuby, FR; red) was evaluated in fluorescence preparations using confocal microscopy. Instances of BDA- (arrows) and FR-labeled (arrowhead) terminals were found to make appositions onto single PVH-projecting neurons in aBST, by analysis of single optical planes containing fluorescence labeling for all three markers. Bottom row: After a single stress exposure, numerous instances of Fos-labeled nuclei are evident in PVH-projecting neurons containing appositions from BDA- (left) and FR-labeled (right) terminals. Data are based upon Radley and Sawchenko (2011).