Fig. 2.

Localization of APJ mRNA in the peripheral tissues of the adult mouse by ISHH. Low magnification images of autoradiographic films of slide-mounted tissue sections hybridized with an antisense ³⁵S-labeled APJ riboprobe. (A) The kidney exhibits labeling in the outer cortex (c) and the medulla (m). (B) In the lung there is strong labeling throughout the parenchyma. (C) The pyloric region of the stomach is shown with specific labeling in the mucosa muscularis (mm) and the duodenum indicated (d). (D) At higher magnification, specific labeling in the villi (v) and mucosa (mm) of the ileum is observed. (E) Labeling in the heart atrium (a) and ventricle (v) with corresponding sense ³⁵S-labeled APJ riboprobe shown (inset). (F) An absence of signal was observed in the APJ KO heart atrium (a) and ventricle (v). All scale bars equal 1 mm.