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. 2012 Feb 16;11(2):129–139. doi: 10.1016/j.chom.2012.01.006

Figure 1.

Figure 1

WRC and N-WASP Recruitment and Activation by SopE

(A) Proteins recruited from extract to phospholipid bilayers by SopE. Silica beads coated with phospholipid bilayers (PL) alone (control) or decorated with SopE (PL +SopE) isolated from brain extract in the presence or absence of GTPγS. Recruited proteins were extracted, analyzed by SDS-PAGE and Coomassie blue staining. Proteins from gel bands marked with orange circles were identified (labeled right) by mass spectrometry. Membrane-bound SopE (green circle). Molecular weight markers in kDa (left).

(B) Parallel immunoblotting of samples from (A) with indicated antibodies (right). See also Table S1.

(C) Fluorescence microscopy of rhodamine-actin assembly on PL beads decorated with SopE (PL +SopE) in extract alone or supplemented with GTPγS, and in extract preincubated with inhibitors PBD and waspΔvca. Scale bar 15 μm.