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. 2012 Feb 16;11(2):129–139. doi: 10.1016/j.chom.2012.01.006

Figure 2.

Figure 2

SopE Cooperation with Arf GTPase in Activation of WRC

(A) Recruitment of Rho and Arf GTPase activation probes to SopE-decorated PL beads. PL beads alone (control) or decorated with SopE (PL +SopE) isolated from brain extract with GTPγS and from extract preincubated with control buffer, PBD, or GAT (orange circles). Membrane-bound SopE (green circle). Molecular weight markers in kDa (left).

(B) Fluorescence microscopy of rhodamine-actin assembly on the PL beads decorated with SopE alone or together with anchored active myristoylated Arf1GTPγS in extract with or without waspΔvca. Scale bar 15 μm. See also Figure S1.