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. 2012 Feb 16;11(2):129–139. doi: 10.1016/j.chom.2012.01.006

Figure 6.

Figure 6

WRC Recruitment by ARNO

(A) Proteins recruited from extract to phospholipid bilayers by ARNO. PL beads decorated with ARNO (PL +ARNO) isolated from brain extract in the presence or absence of GTPγS. Recruited proteins were extracted, analyzed by SDS-PAGE and Coomassie blue staining. Proteins from gel bands marked with orange circles were identified (labeled right) by mass spectrometry. Membrane-bound ARNO (green circle). Molecular weight markers in kDa (left).

(B) Parallel immunoblotting of samples from (A) with indicated antibodies (right).

(C) Proteins recruited to PL beads by ARNO from extract containing Rho and Arf GTPase activation probes. PL beads decorated with ARNO (PL +ARNO) isolated from brain extract supplemented with GTPγS and from extract preincubated with control buffer, PBD, or GAT (orange circles). Membrane-bound ARNO (green circle). Molecular weight markers in kDa (left).

(D) PL beads decorated with either ARNO (samples from C) or SopE (Figure 2A) were analyzed by SDS-PAGE and immunoblotting with indicated antibodies (right). See also Figure S4.