Skip to main content
. 2012 Feb 16;11(2):129–139. doi: 10.1016/j.chom.2012.01.006

Figure 7.

Figure 7

Cooperation of SopE and ARNO in Recruitment and Activation of WRC

(A) WRC recruitment to phospholipid bilayers by ARNO and SopE together. PL beads decorated with either ARNO or SopE, or both GEFs together were isolated from brain extract in the absence of GTPγS. Recruited proteins were extracted before analysis by SDS-PAGE and immunoblotting with indicated antibodies (right). See also Figure S4.

(B) Fluorescence microscopy of rhodamine-actin assembly on PL beads from (A) in extract without GTPγS in the presence or absence of waspΔvca, PBD, or GAT. Scale bar 15 μm.

(C) Actin assembly by ARNO and SopE in cells. HeLa cells expressing either SopEFLAG (SopE) or CFPARNO (ARNO) or both GEFs together were fixed, then stained with anti-FLAG to label SopEFLAG and Alexa Fluor 350-phalloidin to label actin. Insets magnify SopE, ARNO, and actin colocalizing at macropinosomes. Scale bar 8 μm.