Fig. 7.

Effect of different levels of noise on the estimation of the release time course. (A–C) Postsynaptic traces of 10 selected events at three simulated synapses with parameters equal to that in Fig. 5A, but with mean peak amplitudes adjusted to obtain SNRs of 4 (A), 3 (B) and 2 (C). (D) RTC estimates obtained with template extraction under the three different SNR conditions using the binomial correction method are plotted in red (traces in A), green (traces in B), blue (traces in C), together with the true underlying RTC they were drawn from (in black). (E) Bias in the estimation of the RTC half-width due to noise, calculated as in Fig. 6; n_UQM, fraction of uniquantal events missed due to the noise level. (F) Bias when the noise is present together with all problems described in Fig. 6, with E_N = CV_P = n_SM = n_UQM = E_total. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of the article.)