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Pre-steady-state kinetic analysis of DHBV-RT pyrophosphorolysis. (a) A time course of [α-32P]dCMP removal from the viral primer terminus was used to estimate the pyrophosphorolysis rate constant. (b) The PPi levels were varied to estimate the PPi concentration dependence of pyrophosphorolysis. A time course was also determined for individual PPi concentrations to ensure that the reactions did not plateau before reaction termination.
