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PPi exchange activity of DHBV cores (a) and avian myeloblastosis virus (AMV)-RT (b) determined under identical conditions. After a 30-min incubation with dNTPs and 32PPi, reaction mixtures were separated by TLC, the products quantified, and data plotted reaction velocity versus PPi (c) for DHBV (♦) and AMV-RT (■). Although dAMP was removed ≈5-fold more efficiently than other dNMPs by both RTs, this disparity was not observed when lower, physiological concentrations of dNTPs were used.
