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Steady-state reversible inhibition of DHBV DNA synthesis by PPi. The effect of 0, 50, 100, and 250 μM PPi on DHBV-RT DNA synthesis was determined under varying dNTP concentrations. After 60 min at 37°C, the reactions were terminated, and the reciprocal of the reaction velocity was plotted versus the reciprocal of substrate concentration (Lineweaver-Burk plot).
