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. 2012 Feb;17-318(2-12):113–121. doi: 10.1016/j.tplants.2011.11.004

Figure 3.

Figure 3

Analysis of various gene expression patterns in different Arabidopsis organs. (a)ATHB8[98,99] transcript accumulation in procambial strands (arrowheads) as visualized by RNA in situ hybridization indicates the early initiation of vascular tissue in lateral organs. A longitudinal section through a generative apex is shown. The asterisk denotes the SAM. (b) As indicated by the activity of a STM:GUS reporter [100], the STM promoter is active in differentiated tissues of vascular bundles in stems. (c) The SCR:GFP marker visualizes the starch sheath at the bottom of stems of 15-cm-tall plants (green signal). The rough hand section was counterstained with propidium iodide (red signal). Asterisks label the position of primary vascular bundles. (d, e) RNA in situ hybridizations of vegetative shoot apices reveal an early activation of WOX4 (d) in vascular bundles of leaf primordia which is, however, later than the activation of the ‘preprocambial’ marker ATHB8(e). Arrows indicate the youngest primordia in which respective transcript accumulation is detectable. Asterisks label the SAM. Scale bars: 100 μm. Same magnification in (d) and (e). RNA in situ hybridizations were performed as described previously [71]. Abbreviations: FC, Fascicular cambium; FP, flower primordium; IF, interfascicular fibers; Ph, phloem; SS, starch sheath; Xy, xylem.