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. 2012 Feb 1;11(3):479–488. doi: 10.4161/cc.11.3.18994

Figure 1.

Figure 1

Generation of SGO1 mutant mice. (A) Schematic representation of knockout construct. Disruption of the SGO1 locus was obtained by a gene trap method. LTR, viral long-terminal repeat; SA, splice acceptor; NEO, neomycin; pA, poly adenylation sequence. (B) Structure of the wild-type and mutant SGO1 loci. *denotes the gene trap cassette insertion site. (C) Representative genotyping result by PCR. WT, wild type; HT, heterozygous. PCR products were run on 1% agarose gels. The WT allele generates a 423 bp PCR product, while the mutant allele generates a 280 bp product. (D) Relative mRNA level of SGO1 from mice tail tissue. mRNA levels were measured by quantitative-RT-PCR. The average tissue mRNA level of WT mice was arbitrarily set to 1, and the tissue mRNA level of HT mice was divided by the WT average level (n = 3/group). (E) Western blot analysis of SGO1 levels in tail tissue. (F) The signals shown in the western blot film were quantified by densitometry.