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. 2012 Feb 1;11(3):479–488. doi: 10.4161/cc.11.3.18994

Figure 4.

Figure 4

SGO1+/− mice show enhanced acute colonic cell death with AOM treatments. (A) Schematic presentation of mouse tumorigenesis assay. At 7 weeks of age, mice were grouped, and starting at 8 weeks of age, they were given s.c. azoxymethane (AOM, 4 mg/kg body weight) two times weekly for 4 weeks. Asterisks indicate timing for FACS sample harvest to assess acute response to AOM on 1, 3 and 5 weeks after completion of AOM treatments. Twelve weeks after completion of AOM treatment, all remaining mice were sacrificed, and their colons were removed for FACS, immunoblotting and ACF/adenoma counting. (B) Colonic mucosa in SGO1+/− mice show a higher acute cell death rate in response to AOM treatments. Cell death rate (i.e., percentage of cells with sub-G1 DNA) is estimated with FACS analysis of colonic mucosa in AOM-treated wild-type and SGO1+/− mice. Cell death rate in untreated wild-type is less than 10%.