Figure 6.
Differential marker protein expressions in wild-type and SGO1+/− mice. (A) Extracts of colonic mucosa from three wild-type and three SGO1+/− mice were subjected to immunoblotting. All samples are from the endpoint (12 weeks after completion of AOM treatment). α-tubulin used as loading control. (B) Immunohistochemistry of endpoint samples for the overexpressed proteins (Bcl-2, left column; COX2, middle column; p53, right column) counterstained with Hematoxylin. Upper part: normal-looking colonic crypts from wild type. Lower part: normal-looking colonic crypts from SGO+/− mice.