Figure 4.

Inhibition of Cdc6 during maturation causes abnormal spindle assembly. Stage VI oocytes were injected with various concentrations of Cdc6 antisense or control non-specific oligonucleotides, treated with progesterone to induce maturation and samples were collected during early MI (between 30–60 min post-GVBD) and stained for α tubulin (red), Cdc6 (green) and DNA (blue). (A and B) At 110–150 ng of antisense injection, multiple spindles, tripolar spindles were noted. (C and D) At 170–190 ng of antisense injection, elongated, distorted spindle-like structure or tubulin mass attached with condensed chromosomes were seen. (E) Control non-specific oligonucleotides injected (210 ng) oocytes had normal prometaphase MI spindle. Image scale: 65–70 µm.