Figure 5.

Cdc6 co-localizes with γ-tubulin at interphase centrosomes of mammalian cells. Affinity-purified antibodies to Cdc6 protein stain puncta at the perinuclear focus of microtubule staining (the centrosome) in a variety of mammalian cell lines, including HeLa cells [shown here in (B)]. At either lower (B) or higher (C) magnification, the colocalization of Cdc6 with one (or sometimes both) puncta of centrosomes (stained with antibodies to gamma tubulin) is observed. Cdc6 stained red; γ-tubulin (C) or α-tubulin (B) stained green, and DNA stained blue with Hoechst. (A) is the phase contrast image of HeLa cells. Scale bars in (A and B = 5 micron) and (C = 1 micron).