Figure 2.

The resynchronization kinetics of circadian Bmal1-luciferase expression by simulated body temperature cycles depend on the old phase. NIH3T3/Bmal1-luc cells were transiently synchronized with horse serum and then subjected to temperature cycles starting 24, 30, 36, or 42 h after the serum treatment (phases 1, 2, 3, and 4). The top left panel shows bioluminescence from a cell population kept at constant temperature. The next four panels show the responses of cells subjected to temperature phases 1, 2, 3, and 4. All bioluminescence data were filtered by moving average transformation.