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. Author manuscript; available in PMC: 2012 Mar 29.
Published in final edited form as: Neuroscience. 2010 Feb 24;167(3):786–798. doi: 10.1016/j.neuroscience.2010.02.037

Table 4.

Number of mRNA molecules in a single cultured hippocampal neuron

CaV subunit Number of transcript molecules
CaV1.1 0.0
CaV1.2 5.0
CaV1.3 2.2
CaV1.4 0.0
CaV2.1 11.2
CaV2.2 5.4
CaV2.3 2.3
β1 3.6
β2 4.2
β3 4.4
β4 3.8
α2δ-1 2.3
α2δ-2 1.8
α2δ-3 2.1
α2δ-4a 0.0

Calculations are based on the qRT-PCR experiment on cultured hippocampal neurons (24 DIV) with highest RNA yield.

a

The assay for α2δ-4 was not included in the same qRT-PCR experiments. However, in other qRT-PCR runs on cultured hippocampal neurons α2δ-4 levels were always below detectability (cf. Fig. 4 and Suppl. Fig. 2).