Table 4.
Number of mRNA molecules in a single cultured hippocampal neuron
CaV subunit | Number of transcript molecules |
---|---|
CaV1.1 | 0.0 |
CaV1.2 | 5.0 |
CaV1.3 | 2.2 |
CaV1.4 | 0.0 |
CaV2.1 | 11.2 |
CaV2.2 | 5.4 |
CaV2.3 | 2.3 |
β1 | 3.6 |
β2 | 4.2 |
β3 | 4.4 |
β4 | 3.8 |
α2δ-1 | 2.3 |
α2δ-2 | 1.8 |
α2δ-3 | 2.1 |
α2δ-4a | 0.0 |
Calculations are based on the qRT-PCR experiment on cultured hippocampal neurons (24 DIV) with highest RNA yield.
The assay for α2δ-4 was not included in the same qRT-PCR experiments. However, in other qRT-PCR runs on cultured hippocampal neurons α2δ-4 levels were always below detectability (cf. Fig. 4 and Suppl. Fig. 2).