Figure 8.

Voltage dependence of TMEM16B mutants. Normalized conductances at the indicated [Ca²⁺]_i calculated from tail currents at −100 mV after prepulses between −200 and +200 mV were plotted versus the prepulse voltage. Black lines are the fit to the Boltzmann equation (Eq. 2) for WT from Fig. 4 at 100 µM Ca²⁺ (solid line) or at 1.5 µM Ca²⁺ (dashed line). Colored lines are the fits to the Boltzmann equation for E367Q (A; n = 3–4), ΔE₅ (B; n = 3–5), and ΔEYE (C; n = 3–6) mutants. (D) Average V_1/2 values plotted versus [Ca²⁺]_i.