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. Author manuscript; available in PMC: 2013 Mar 27.
Published in final edited form as: Biochemistry. 2012 Mar 15;51(12):2425–2435. doi: 10.1021/bi300101r

Figure 4. Photoincorporation of [125I]-SADU-3-72 into the Torpedo nAChR in the absence and presence of Carb.

Figure 4

A, An autoradiograph (12–24 h exposure with intensifying screen) of an 8% SDS-PAGE gel containing native Torpedo nAChR-rich membranes photolabeled with [125I]-SADU-3-72 in the absence (−) and/or the presence (+) of the agonist Carbamylcholine (Carb), the competitive antagonist α-bungarotoxin (αBgTx), the resting state-selective channel blocker tetracaine, or the desensitized state-selective channel blocker thienycyclohexylpiperidine (TCP). The migration of individual nAChR subunits and the alpha subunit of Na/K ATPase (αNK) is indicated on the left. B, 125I cpm incorporation into each nAChR subunit for each of the labeling conditions of Panel A, as determined by γ-counting of individual nAChR subunit bands excised from the dried gel after autoradiography (n =1). The notations −/−, +/−, −/+ and +/+ indicate [125I]SADU-3-72 photolabelings in the presence of αBgTx (resting state), Carb (desensitized sate), αBgTx and tetracaine or Carb and TCP, respectively.